RESUMO
Porcine factor VIII (FVIII; Hyate:C; Speywood Biopharm Ltd, UK) has been used since 1980 for the treatment both of patients with acquired haemophilia and those with congenital haemophilia and inhibitory antibodies. Each batch is extensively screened using cell-culture techniques to confirm the absence of viruses. The production process does not incorporate specific virucidal treatment steps, such as heat treatment or the addition of a solvent/detergent mixture. Low levels of porcine parvovirus were detected in some batches of the product in late 1996 and supply was suspended. In this retrospective study, sera from 81 recipients of porcine FVIII and 125 other volunteers were screened for evidence of antibodies against a range of porcine viruses: porcine parvovirus (PPV), encephalomyocarditis virus (EMCV), and porcine respiratory and reproductive syndrome virus (PRRSV). The 125 volunteer controls included subjects from six categories: healthy control subjects, pig abattoir personnel, personnel involved in the manufacture of porcine FVIII, recipients of porcine heparin, recipients of porcine insulin, and haemophiliacs treated only with human FVIII. No antibodies to PPV or PRRSV were detected in any subject. Four patients and two volunteers were found to have antibodies to EMCV, but this incidence is similar to that observed in the general population. In conclusion, there was no evidence of transmission of PPR or other marker porcine virus associated with the use of porcine FVIII concentrate (Hyate: C).
Assuntos
Contaminação de Medicamentos , Fator VIII/uso terapêutico , Hemofilia A/tratamento farmacológico , Parvoviridae/isolamento & purificação , Animais , Anticorpos Antivirais/sangue , Hemofilia A/virologia , Humanos , Masculino , Parvoviridae/imunologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/imunologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/isolamento & purificação , Estudos Retrospectivos , SuínosRESUMO
An enzyme-linked immunosorbent assay for porcine parvovirus was developed for laboratory detection of parvovirus antigen in fetal tissues and compared with the fluorescent antibody and haemagglutination tests. The ELISA proved to be superior in terms of specificity, sensitivity, speed of performance and ease of interpretation.
Assuntos
Ensaio de Imunoadsorção Enzimática/métodos , Parvoviridae/isolamento & purificação , Virologia/métodos , Animais , Ensaio de Imunoadsorção Enzimática/estatística & dados numéricos , Estudos de Avaliação como Assunto , Doenças Fetais/diagnóstico , Doenças Fetais/veterinária , Feto/microbiologia , Imunofluorescência/estatística & dados numéricos , Testes de Hemaglutinação/estatística & dados numéricos , Infecções por Parvoviridae/diagnóstico , Infecções por Parvoviridae/veterinária , Sensibilidade e Especificidade , Suínos , Doenças dos Suínos/diagnóstico , Virologia/estatística & dados numéricosRESUMO
The transmission of maedi-visna (MV) virus was studied within two groups of sheep, one of which was also affected with sheep pulmonary adenomatosis (SPA). Serological monitoring of sheep kept in contact with both groups indicated that MV virus replication occurred to a greater extent in the group with both diseases, three of five in-contact sheep being seropositive after 1 year's exposure compared with none of six held with MV-virus-only infected lambs.
